Blood-feeding and immunogenic Aedes aegypti saliva proteins

2010

Article référencé dans le Web of Science WOS:000278494100001

Wasinpiyamongkol L., Patramool Sirilaksana, Luplertlop N., Surasombatpattana P., Doucoure Souleymane, Mouchet François, Séveno M., Remoué Franck, Demettre E., Brizard Jean-Paul, Jouin P., Biron D.G., Thomas F., Missé Dorothée

Proteomics, 2010, 10 (10), p. 1906-1916 ISSN 1615-9861

Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal.

Biochimie [020BIOCHI] ; Maladies transmissibles [052CULARB04]

DENGUE ; VECTEUR ; MOUSTIQUE ; VIRUS ; TRANSMISSION ; APPAREIL DIGESTIF ; REPAS ; SANG ; PHYSIOLOGIE ; METABOLISME ; PROTEINE ; IMMUNITE ; REPAS DE SANG ; PROTEOMIQUE ; EXPRESSION DES GENES ; GLANDE SALIVAIRE

MONDE

Fonds IRD [F B010049804]

fdi:010049804