%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Wasinpiyamongkol, L.
%A Patramool, Sirilaksana
%A Luplertlop, N.
%A Surasombatpattana, P.
%A Doucoure, Souleymane
%A Mouchet, François
%A Séveno, M.
%A Remoué, Franck
%A Demettre, E.
%A Brizard, Jean-Paul
%A Jouin, P.
%A Biron, D.G.
%A Thomas, F.
%A Missé, Dorothée
%T Blood-feeding and immunogenic Aedes aegypti saliva proteins
%D 2010
%L fdi:010049804
%G ENG
%J Proteomics
%@ 1615-9861
%K DENGUE ; VECTEUR ; MOUSTIQUE ; VIRUS ; TRANSMISSION ; APPAREIL DIGESTIF ; REPAS ; SANG ; PHYSIOLOGIE ; METABOLISME ; PROTEINE ; IMMUNITE
%K REPAS DE SANG ; PROTEOMIQUE ; EXPRESSION DES GENES ; GLANDE SALIVAIRE
%K MONDE
%M ISI:000278494100001
%N 10
%P 1906-1916
%R 10.1002/pmic.200900626
%U https://www.documentation.ird.fr/hor/fdi:010049804
%> https://www.documentation.ird.fr/intranet/publi/depot/2010-06-01/010049804.pdf
%V 10
%W Horizon (IRD)
%X Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal.
%$ 052CULARB04 ; 020BIOCHI