Wasinpiyamongkol L. auteur aut IRD Patramool Sirilaksana auteur aut IRD Luplertlop N. auteur aut IRD Surasombatpattana P. auteur aut IRD Doucoure Souleymane auteur aut IRD Mouchet François auteur aut IRD Séveno M. auteur aut IRD Remoué Franck auteur aut IRD Demettre E. auteur aut IRD Brizard Jean-Paul auteur aut IRD Jouin P. auteur aut IRD Biron D.G. auteur aut IRD Thomas F. auteur aut IRD Missé Dorothée auteur aut IRD text journalArticle eng text/pdf born digital access Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal. specialized DENGUE VECTEUR MOUSTIQUE VIRUS TRANSMISSION APPAREIL DIGESTIF REPAS SANG PHYSIOLOGIE METABOLISME PROTEINE IMMUNITE REPAS DE SANG PROTEOMIQUE EXPRESSION DES GENES GLANDE SALIVAIRE MONDE 052CULARB04 020BIOCHI 10 10 1906-1916 2010 1615-9861 https://www.documentation.ird.fr/hor/fdi:010049804 10.1002/pmic.200900626 1615-9861 [F B010049804] https://www.documentation.ird.fr/hor/fdi:010049804 https://www.documentation.ird.fr/intranet/publi/depot/2010-06-01/010049804.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2010-06-01 2011-10-19 fdi:010049804 fre