Blood-feeding and immunogenic Aedes aegypti saliva proteins Wasinpiyamongkol, L. /Patramool, Sirilaksana Luplertlop, N. Surasombatpattana, P. /Doucoure, Souleymane /Mouchet, François Séveno, M. /Remoué, Franck Demettre, E. /Brizard, Jean-Paul Jouin, P. Biron, D.G. Thomas, F. /Missé, Dorothée DENGUE VECTEUR MOUSTIQUE VIRUS TRANSMISSION APPAREIL DIGESTIF REPAS SANG PHYSIOLOGIE METABOLISME PROTEINE IMMUNITE REPAS DE SANG PROTEOMIQUE EXPRESSION DES GENES GLANDE SALIVAIRE Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal. 2010 text https://www.documentation.ird.fr/hor/fdi:010049804 fdi:010049804 Wasinpiyamongkol L., Patramool Sirilaksana, Luplertlop N., Surasombatpattana P., Doucoure Souleymane, Mouchet François, Séveno M., Remoué Franck, Demettre E., Brizard Jean-Paul, Jouin P., Biron D.G., Thomas F., Missé Dorothée. Blood-feeding and immunogenic Aedes aegypti saliva proteins. 2010, 10 (10), 1906-1916 EN MONDE