Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Proteomics 1906-1916 DENGUE VECTEUR MOUSTIQUE VIRUS TRANSMISSION APPAREIL DIGESTIF REPAS SANG PHYSIOLOGIE METABOLISME PROTEINE IMMUNITE REPAS DE SANG PROTEOMIQUE EXPRESSION DES GENES GLANDE SALIVAIRE MONDE 2010 fdi:010049804 ENG Proteomics 1615-9861 ISI:000278494100001 10 10.1002/pmic.200900626 https://www.documentation.ird.fr/hor/fdi:010049804 https://www.documentation.ird.fr/intranet/publi/depot/2010-06-01/010049804.pdf 10 Horizon (IRD) Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal. 052CULARB04 ; 020BIOCHI UR165 / UR016 / UR121 Thaïlande