Martinez-Montero M. E., Martinez J., Engelmann Florent. (2008). Cryopreservation of sugarcane somatic embryos. Cryo-Letters, 29 (3), p. 229-242. ISSN 0143-2044.
Martinez-Montero M. E., Martinez J., Engelmann Florent
Source
Cryo-Letters, 2008,
29 (3), p. 229-242 ISSN 0143-2044
in this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. Droplet-vitrification was the most efficient technique. Optimal conditions included loading with a solution containing 1.5 M glycerol and 0.3 M sucrose for 30 min at 25 degrees C, treatment with the PVS2 solution for 20-40 min at 0 degrees C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. Under such conditions, viability of cryopreserved somatic embryos reached 55 %.
