%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Martinez-Montero, M. E.
%A Martinez, J.
%A Engelmann, Florent
%T Cryopreservation of sugarcane somatic embryos
%D 2008
%L fdi:010042701
%G ENG
%J Cryo-Letters
%@ 0143-2044
%K Somatic embryos ; Sugarcane ; Vitrification ; Encapsulation-vitrification ; Droplet-vitrification
%M ISI:000257824900006
%N 3
%P 229-242
%U https://www.documentation.ird.fr/hor/fdi:010042701
%> https://www.documentation.ird.fr/intranet/publi/2008/08/010042701.pdf
%V 29
%W Horizon (IRD)
%X in this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. Droplet-vitrification was the most efficient technique. Optimal conditions included loading with a solution containing 1.5 M glycerol and 0.3 M sucrose for 30 min at 25 degrees C, treatment with the PVS2 solution for 20-40 min at 0 degrees C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. Under such conditions, viability of cryopreserved somatic embryos reached 55 %.
%$ 076