@article{fdi:010042701,
  title = {{C}ryopreservation of sugarcane somatic embryos},
  author = {{M}artinez-{M}ontero, {M}. {E}. and {M}artinez, {J}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {in this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. {V}iability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. {D}roplet-vitrification was the most efficient technique. {O}ptimal conditions included loading with a solution containing 1.5 {M} glycerol and 0.3 {M} sucrose for 30 min at 25 degrees {C}, treatment with the {PVS}2 solution for 20-40 min at 0 degrees {C} followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. {U}nder such conditions, viability of cryopreserved somatic embryos reached 55 %.},
  keywords = {{S}omatic embryos ; {S}ugarcane ; {V}itrification ; {E}ncapsulation-vitrification ; {D}roplet-vitrification},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {29},
  numero = {3},
  pages = {229--242},
  ISSN = {0143-2044},
  year = {2008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042701},
}