Martinez-Montero M. E. auteur aut IRD Martinez J. auteur aut IRD Engelmann Florent auteur aut IRD text journalArticle eng text/pdf born digital access in this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. Droplet-vitrification was the most efficient technique. Optimal conditions included loading with a solution containing 1.5 M glycerol and 0.3 M sucrose for 30 min at 25 degrees C, treatment with the PVS2 solution for 20-40 min at 0 degrees C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. Under such conditions, viability of cryopreserved somatic embryos reached 55 %. specialized Somatic embryos Sugarcane Vitrification Encapsulation-vitrification Droplet-vitrification 076 29 3 229-242 2008 0143-2044 https://www.documentation.ird.fr/hor/fdi:010042701 0143-2044 [F B010042701] https://www.documentation.ird.fr/hor/fdi:010042701 https://www.documentation.ird.fr/intranet/publi/2008/08/010042701.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-09-19 2017-08-23 fdi:010042701 fre