Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Cryo-Letters 229-242 Somatic embryos Sugarcane Vitrification Encapsulation-vitrification Droplet-vitrification 2008 fdi:010042701 ENG Cryo-Letters 0143-2044 ISI:000257824900006 3 https://www.documentation.ird.fr/hor/fdi:010042701 https://www.documentation.ird.fr/intranet/publi/2008/08/010042701.pdf 29 Horizon (IRD) in this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. Droplet-vitrification was the most efficient technique. Optimal conditions included loading with a solution containing 1.5 M glycerol and 0.3 M sucrose for 30 min at 25 degrees C, treatment with the PVS2 solution for 20-40 min at 0 degrees C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. Under such conditions, viability of cryopreserved somatic embryos reached 55 %. 076 UR188 Cuba