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Nguema P. P. M., Onanga R., Atome G. R. N., Mbeang J. C. O., Mabika A. M., Yaro M., Lounnas Manon, Dumont Y., Zohra Z. F., Godreuil S., Bretagnolle F. (2020). Characterization of ESBL-producing enterobacteria from fruit bats in an unprotected area of Makokou, Gabon [+ Correction in : Microorganisms, 2020, vol.8, no 9, art. 1384]. Microorganisms, 8 (1), art. 138 [15 p. + Correction in : Microorganisms, 2020, vol.8, no 9, art. 1384].

Titre du document
Characterization of ESBL-producing enterobacteria from fruit bats in an unprotected area of Makokou, Gabon [+ Correction in : Microorganisms, 2020, vol.8, no 9, art. 1384]
Année de publication
2020
Type de document
Article référencé dans le Web of Science WOS:000514650700052
Auteurs
Nguema P. P. M., Onanga R., Atome G. R. N., Mbeang J. C. O., Mabika A. M., Yaro M., Lounnas Manon, Dumont Y., Zohra Z. F., Godreuil S., Bretagnolle F.
Source
Microorganisms, 2020, 8 (1), art. 138 [15 p. + Correction in : Microorganisms, 2020, vol.8, no 9, art. 1384]
In Gabon, terrestrial mammals of protected areas have been identified as a possible source of antibiotic-resistant bacteria. Some studies on antibiotic resistance in bats have already been carried out. The main goal of our study was to detect extended-spectrum beta-lactamases (ESBLs) that are produced by enterobacteria from bats in the Makokou region in Gabon. Sixty-eight fecal samples were obtained from 68 bats caught in the forests located 1 km from the little town of Makokou. After culture and isolation, 66 Gram-negative bacterial colonies were obtained. The double-disk diffusion test confirmed the presence of ESBLs in six (20.69%) Escherichia coli isolates, four (13.79%) Klebsiella pneumoniae isolates, and one (3.45%) Enterobacter cloacae isolate. The analysis based on the nucleotide sequences of the ESBL resistance genes showed that all cefotaximase-Munichs (CTX-Ms) were CTX-M-15 and that all sulfhydryl variables (SHVs) were SHV-11: 41.67% CTX-M-15-producing E. coli, 16.67% CTX-M-15+SHV-11-producing E. coli, 8.33% CTX-M-15-producing K. pneumoniae, 25% CTX-M-15+SHV-11-producing K. pneumoniae, and 8.33% CTX-M-15-produced E. cloacae. This study shows for the first time the presence of multiresistant ESBL-producing enterobacteria in fruit bats in Makokou.
Plan de classement
Sciences du monde animal [080] ; Biotechnologies [084]
Description Géographique
GABON
Localisation
Fonds IRD [F B010077967]
Identifiant IRD
fdi:010077967
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