Holzmuller P., Biron D. G., Courtois P., Koffi M., Bras Goncalves Rachel, Daulouede S., Solano Philippe, Cuny Gérard, Vincendeau P., Jamonneau Vincent. (2008). Virulence and pathogenicity patterns of Trypanosoma brucei gambiense field isolates in experimentally infected mouse : differences in host immune response modulation by secretome and proteomics. Microbes and Infection, 10 (1), p. 79-86. ISSN 1286-4579.
Titre du document
Virulence and pathogenicity patterns of Trypanosoma brucei gambiense field isolates in experimentally infected mouse : differences in host immune response modulation by secretome and proteomics
Holzmuller P., Biron D. G., Courtois P., Koffi M., Bras Goncalves Rachel, Daulouede S., Solano Philippe, Cuny Gérard, Vincendeau P., Jamonneau Vincent
Source
Microbes and Infection, 2008,
10 (1), p. 79-86 ISSN 1286-4579
Human African trypanosomiasis is characterised by an important clinical diversity. Although Trypanosoma brucei gambiense field stocks isolated from patients in the same focus did not exhibit apparent genetic variability, they showed marked differences in terms of virulence (capacity to multiply inside a host) and pathogenicity (ability of producing mortality) in experimental murine infections. Two strains exhibiting opposite pathogenic and virulence properties in mouse were further investigated through their host-parasite interactions. In vitro, parasite bloodstream forms or soluble factors (or secretome) from both strains induced macrophage arginase as a function of their virulence. Arginase expression, a hallmark of macrophage alternative activation pathway, favours trypanosome bloodstream forms development. Moreover, a comparative proteomic study of the trypanosome stocks' secretomes evidenced both a differential expression of common molecules and the existence of stock specific molecules. This highlighted the potential involvement of the differential expression of the same genome in the diverse infectious properties of trypanosomes.
