%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Holzmuller, P.
%A Biron, D. G.
%A Courtois, P.
%A Koffi, M.
%A Bras Goncalves, Rachel
%A Daulouede, S.
%A Solano, Philippe
%A Cuny, Gérard
%A Vincendeau, P.
%A Jamonneau, Vincent
%T Virulence and pathogenicity patterns of Trypanosoma brucei gambiense field isolates in experimentally infected mouse : differences in host immune response modulation by secretome and proteomics
%D 2008
%L fdi:010042524
%G ENG
%J Microbes and Infection
%@ 1286-4579
%K trypanosoma brucei gambiense ; virulence ; pathogenicity ; parasite soluble factors ; immunomodulation ; proteomics
%M CC:0002543725-0012
%N 1
%P 79-86
%R 10.1016/j.micinf.2007.10.008
%U https://www.documentation.ird.fr/hor/fdi:010042524
%> https://www.documentation.ird.fr/intranet/publi/2008/04/010042524.pdf
%V 10
%W Horizon (IRD)
%X Human African trypanosomiasis is characterised by an important clinical diversity. Although Trypanosoma brucei gambiense field stocks isolated from patients in the same focus did not exhibit apparent genetic variability, they showed marked differences in terms of virulence (capacity to multiply inside a host) and pathogenicity (ability of producing mortality) in experimental murine infections. Two strains exhibiting opposite pathogenic and virulence properties in mouse were further investigated through their host-parasite interactions. In vitro, parasite bloodstream forms or soluble factors (or secretome) from both strains induced macrophage arginase as a function of their virulence. Arginase expression, a hallmark of macrophage alternative activation pathway, favours trypanosome bloodstream forms development. Moreover, a comparative proteomic study of the trypanosome stocks' secretomes evidenced both a differential expression of common molecules and the existence of stock specific molecules. This highlighted the potential involvement of the differential expression of the same genome in the diverse infectious properties of trypanosomes.
%$ 052