Russell A. J. C., Sanderson T., Bushell E., Talman Arthur, Anar B., Girling G., Hunziker M., Kent R. S., Martin J. S., Metcalf T., Montandon R., Pandey V., Pardo M., Roberts A. B., Sayers C., Schwach F., Choudhary J. S., Rayner J. C., Voet T., Modrzynska K. K., Waters A. P., Lawniczak M. K. N., Billker O. (2023). Regulators of male and female sexual development are critical for the transmission of a malaria parasite. Cell Host and Microbe, 31 (2), p. 305-319.e1-e10. ISSN 1931-3128.
Titre du document
Regulators of male and female sexual development are critical for the transmission of a malaria parasite
Année de publication
2023
Auteurs
Russell A. J. C., Sanderson T., Bushell E., Talman Arthur, Anar B., Girling G., Hunziker M., Kent R. S., Martin J. S., Metcalf T., Montandon R., Pandey V., Pardo M., Roberts A. B., Sayers C., Schwach F., Choudhary J. S., Rayner J. C., Voet T., Modrzynska K. K., Waters A. P., Lawniczak M. K. N., Billker O.
Source
Cell Host and Microbe, 2023,
31 (2), p. 305-319.e1-e10 ISSN 1931-3128
Malaria transmission to mosquitoes requires a developmental switch in asexually dividing blood-stage par-asites to sexual reproduction. In Plasmodium berghei, the transcription factor AP2-G is required and suffi-cient for this switch, but how a particular sex is determined in a haploid parasite remains unknown. Using a global screen of barcoded mutants, we here identify genes essential for the formation of either male or fe-male sexual forms and validate their importance for transmission. High-resolution single-cell transcriptomics of ten mutant parasites portrays the developmental bifurcation and reveals a regulatory cascade of putative gene functions in the determination and subsequent differentiation of each sex. A male-determining gene with a LOTUS/OST-HTH domain as well as the protein interactors of a female-determining zinc-finger protein indicate that germ-granule-like ribonucleoprotein complexes complement transcriptional processes in the regulation of both male and female development of a malaria parasite.
Localisation
Fonds IRD [F B010087601]
Identifiant IRD
fdi:010087601
