%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Russell, A. J. C.
%A Sanderson, T.
%A Bushell, E.
%A Talman, Arthur
%A Anar, B.
%A Girling, G.
%A Hunziker, M.
%A Kent, R. S.
%A Martin, J. S.
%A Metcalf, T.
%A Montandon, R.
%A Pandey, V.
%A Pardo, M.
%A Roberts, A. B.
%A Sayers, C.
%A Schwach, F.
%A Choudhary, J. S.
%A Rayner, J. C.
%A Voet, T.
%A Modrzynska, K. K.
%A Waters, A. P.
%A Lawniczak, M. K. N.
%A Billker, O.
%T Regulators of male and female sexual development are critical for the transmission of a malaria parasite
%D 2023
%L fdi:010087601
%G ENG
%J Cell Host and Microbe
%@ 1931-3128
%M ISI:000964163800001
%N 2
%P 305-319.e1-e10
%R 10.1016/j.chom.2022.12.011
%U https://www.documentation.ird.fr/hor/fdi:010087601
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-06/010087601.pdf
%V 31
%W Horizon (IRD)
%X Malaria transmission to mosquitoes requires a developmental switch in asexually dividing blood-stage par-asites to sexual reproduction. In Plasmodium berghei, the transcription factor AP2-G is required and suffi-cient for this switch, but how a particular sex is determined in a haploid parasite remains unknown. Using a global screen of barcoded mutants, we here identify genes essential for the formation of either male or fe-male sexual forms and validate their importance for transmission. High-resolution single-cell transcriptomics of ten mutant parasites portrays the developmental bifurcation and reveals a regulatory cascade of putative gene functions in the determination and subsequent differentiation of each sex. A male-determining gene with a LOTUS/OST-HTH domain as well as the protein interactors of a female-determining zinc-finger protein indicate that germ-granule-like ribonucleoprotein complexes complement transcriptional processes in the regulation of both male and female development of a malaria parasite.