Nana-Djeunga H. C., Sicard C. M., Mogoung-Wafo A. E., Chesnais Cédric, Deleglise H., Touka-Nounkeu R., Domche A., Golden A., Klion A. D., Nutman T. B., Boussinesq Michel, Kamgno J., Pion Sébastien. (2022). Changes in onchocerciasis Ov16 IgG4 rapid diagnostic test results over one-month follow-up : lessons for reading timeframe and decision-making. American Journal of Tropical Medicine and Hygiene, 107 (3), p. 658-661. ISSN 0002-9637.
Titre du document
Changes in onchocerciasis Ov16 IgG4 rapid diagnostic test results over one-month follow-up : lessons for reading timeframe and decision-making
Année de publication
2022
Auteurs
Nana-Djeunga H. C., Sicard C. M., Mogoung-Wafo A. E., Chesnais Cédric, Deleglise H., Touka-Nounkeu R., Domche A., Golden A., Klion A. D., Nutman T. B., Boussinesq Michel, Kamgno J., Pion Sébastien
Source
American Journal of Tropical Medicine and Hygiene, 2022,
107 (3), p. 658-661 ISSN 0002-9637
The SD Bioline (R) IgG4 rapid diagnostic test (RDT) detects IgG4 antibodies induced by the Onchocerca vol-vulus-specific antigen Ov16. We evaluated the stability of the RDT results over 1 month, at different time points after completion of each assay, using eluted dried blood spots collected in central Cameroon. Agreement coefficients regarding positivity between 30 minutes and 24 hours, 1, 2, 3, and 4 weeks were, 96.4%, 93.4%, 93.3%, 93.2%, and 93.2%, respectively. Between 30 minutes and 24 hours, 3.6% of the 15,444 tests showed inconsistent results with 81.2% of these tests changing from negative to positive, increasing O. volvulus antibody prevalence from 23.9% to 26.2% (P < 0.0001). This change from negative to positive outcome was confirmed at the subsequent timepoints. Depending on the desired accuracy of prevalence estimates, reading time may have to be redefined more strictly.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010086660]
Identifiant IRD
fdi:010086660
