Nana-Djeunga H. C. auteur aut IRD Sicard C. M. auteur aut IRD Mogoung-Wafo A. E. auteur aut IRD Chesnais Cédric auteur aut IRD Deleglise H. auteur aut IRD Touka-Nounkeu R. auteur aut IRD Domche A. auteur aut IRD Golden A. auteur aut IRD Klion A. D. auteur aut IRD Nutman T. B. auteur aut IRD Boussinesq Michel auteur aut IRD Kamgno J. auteur aut IRD Pion Sébastien auteur aut IRD text journalArticle eng text/pdf reformatted digital access The SD Bioline (R) IgG4 rapid diagnostic test (RDT) detects IgG4 antibodies induced by the Onchocerca vol-vulus-specific antigen Ov16. We evaluated the stability of the RDT results over 1 month, at different time points after completion of each assay, using eluted dried blood spots collected in central Cameroon. Agreement coefficients regarding positivity between 30 minutes and 24 hours, 1, 2, 3, and 4 weeks were, 96.4%, 93.4%, 93.3%, 93.2%, and 93.2%, respectively. Between 30 minutes and 24 hours, 3.6% of the 15,444 tests showed inconsistent results with 81.2% of these tests changing from negative to positive, increasing O. volvulus antibody prevalence from 23.9% to 26.2% (P < 0.0001). This change from negative to positive outcome was confirmed at the subsequent timepoints. Depending on the desired accuracy of prevalence estimates, reading time may have to be redefined more strictly. specialized 050 052 107 3 658-661 2022 0002-9637 https://www.documentation.ird.fr/hor/fdi:010086660 10.4269/ajtmh.21-1201 0002-9637 [F B010086660] https://www.documentation.ird.fr/hor/fdi:010086660 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-01/010086660.pdf IRD - Base Horizon / Pleins textes 2023-01-11 2023-01-11 fdi:010086660 fre