Baronti Cécile, Coutard B., Lamballerie X. de, Charrel R., Touret F. (2021). Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation. Journal of Virological Methods, 297, 114252 [4 p.]. ISSN 0166-0934.
Titre du document
Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying techniques for viruses represent a method of choice for the preservation of strains and their distribution without the need for a demanding cold chain. Here, we describe an optimised lyophilisation protocol usable for SARS-CoV-2 strains that improves preservation and thermostability. We show that sucrose used as an adjuvant represents a simple and efficient stabilizer providing increased protection for long-term preservation and shipment of the virus under different climatic conditions.
