%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Baronti, Cécile
%A Coutard, B.
%A Lamballerie, X. de
%A Charrel, R.
%A Touret, F.
%T Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
%D 2021
%L fdi:010083160
%G ENG
%J Journal of Virological Methods
%@ 0166-0934
%K COVID-19 ; SARS-CoV-2 ; Lyophilisation ; Stabilizers ; Biobanking
%M ISI:000702880600019
%P 114252 [4 ]
%R 10.1016/j.jviromet.2021.114252
%U https://www.documentation.ird.fr/hor/fdi:010083160
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-11/010083160.pdf
%V 297
%W Horizon (IRD)
%X In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying techniques for viruses represent a method of choice for the preservation of strains and their distribution without the need for a demanding cold chain. Here, we describe an optimised lyophilisation protocol usable for SARS-CoV-2 strains that improves preservation and thermostability. We show that sucrose used as an adjuvant represents a simple and efficient stabilizer providing increased protection for long-term preservation and shipment of the virus under different climatic conditions.
%$ 052