Thirion Laurence, Pezzi L., Pedrosa-Corral I., Sanbonmatsu-Gamez S., Lamballerie X. de, Falchi A., Perez-Ruiz M., Charrel R. N. (2021). Evaluation of a Trio Toscana Virus Real-Time RT-PCR assay targeting three genomic regions within nucleoprotein gene. Pathogens, 10 (3), 254 [11 p.].
Titre du document
Evaluation of a Trio Toscana Virus Real-Time RT-PCR assay targeting three genomic regions within nucleoprotein gene
Thirion Laurence, Pezzi L., Pedrosa-Corral I., Sanbonmatsu-Gamez S., Lamballerie X. de, Falchi A., Perez-Ruiz M., Charrel R. N.
Source
Pathogens, 2021,
10 (3), 254 [11 p.]
Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the nucleoprotein gene. Here, they were combined to create a multi-region assay named Trio TOSV RT-qPCR consisting of six primers and three probes. In this study, (i) we evaluated in silico the three RT-qPCR assays available in the literature for TOSV detection, (ii) we combined the three systems to create the Trio TOSV RT-qPCR, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the Trio TOSV RT-qPCR assay, and (iv) we compared the performance of the Trio TOSV RT-qPCR assay with one of the reference monoplex assays on clinical samples. In conclusion, the Trio TOSV RT-qPCR assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Descripteurs
ZONE MEDITERRANEENNE
Description Géographique
ITALIE ; FRANCE ; TUNISIE ; ALGERIE ; PORTUGAL ; ESPAGNE ; MAROC ; CROATIE ; TURQUIE ; GRECE
