@article{fdi:010081167,
  title = {{E}valuation of a {T}rio {T}oscana {V}irus {R}eal-{T}ime {RT}-{PCR} assay targeting three genomic regions within nucleoprotein gene},
  author = {{T}hirion, {L}aurence and {P}ezzi, {L}. and {P}edrosa-{C}orral, {I}. and {S}anbonmatsu-{G}amez, {S}. and {L}amballerie, {X}. de and {F}alchi, {A}. and {P}erez-{R}uiz, {M}. and {C}harrel, {R}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}oscana virus ({TOSV}) can cause central nervous system infections in both residents of and travelers to {M}editerranean countries. {D}ata mining identified three real-time {RT}-q{PCR} assays for detecting {TOSV} {RNA} targeting non-overlapping regions in the nucleoprotein gene. {H}ere, they were combined to create a multi-region assay named {T}rio {TOSV} {RT}-q{PCR} consisting of six primers and three probes. {I}n this study, (i) we evaluated in silico the three {RT}-q{PCR} assays available in the literature for {TOSV} detection, (ii) we combined the three systems to create the {T}rio {TOSV} {RT}-q{PCR}, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the {T}rio {TOSV} {RT}-q{PCR} assay, and (iv) we compared the performance of the {T}rio {TOSV} {RT}-q{PCR} assay with one of the reference monoplex assays on clinical samples. {I}n conclusion, the {T}rio {TOSV} {RT}-q{PCR} assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.},
  keywords = {{ZONE} {MEDITERRANEENNE} ; {P}hlebovirus ; {P}henuiviridae ; meningitis ; sand fly ; {P}hlebotomus ; arbovirus ; {M}editerranean ; diagnostics ; {ITALIE} ; {FRANCE} ; {TUNISIE} ; {ALGERIE} ; {PORTUGAL} ; {ESPAGNE} ; {MAROC} ; {CROATIE} ; {TURQUIE} ; {GRECE}},
  booktitle = {},
  journal = {{P}athogens},
  volume = {10},
  numero = {3},
  pages = {254 [11 ]},
  year = {2021},
  DOI = {10.3390/pathogens10030254},
  URL = {https://www.documentation.ird.fr/hor/fdi:010081167},
}