%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Thirion, Laurence
%A Pezzi, L.
%A Pedrosa-Corral, I.
%A Sanbonmatsu-Gamez, S.
%A Lamballerie, X. de
%A Falchi, A.
%A Perez-Ruiz, M.
%A Charrel, R. N.
%T Evaluation of a Trio Toscana Virus Real-Time RT-PCR assay targeting three genomic regions within nucleoprotein gene
%D 2021
%L fdi:010081167
%G ENG
%J Pathogens
%K ZONE MEDITERRANEENNE
%K Phlebovirus ; Phenuiviridae ; meningitis ; sand fly ; Phlebotomus ; arbovirus ; Mediterranean ; diagnostics
%K ITALIE ; FRANCE ; TUNISIE ; ALGERIE ; PORTUGAL ; ESPAGNE ; MAROC ; CROATIE ; TURQUIE ; GRECE
%M ISI:000633986700001
%N 3
%P 254 [11 ]
%R 10.3390/pathogens10030254
%U https://www.documentation.ird.fr/hor/fdi:010081167
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-05/010081167.pdf
%V 10
%W Horizon (IRD)
%X Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the nucleoprotein gene. Here, they were combined to create a multi-region assay named Trio TOSV RT-qPCR consisting of six primers and three probes. In this study, (i) we evaluated in silico the three RT-qPCR assays available in the literature for TOSV detection, (ii) we combined the three systems to create the Trio TOSV RT-qPCR, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the Trio TOSV RT-qPCR assay, and (iv) we compared the performance of the Trio TOSV RT-qPCR assay with one of the reference monoplex assays on clinical samples. In conclusion, the Trio TOSV RT-qPCR assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.
%$ 052 ; 020