Publications des scientifiques de l'IRD

Laidoudi Y., Medkour H., Levasseur A., Davoust B., Mediannikov Oleg. (2020). New molecular data on Filaria and its Wolbachia from red howler monkeys (Alouatta macconnelli) in French Guiana : a preliminary study. Pathogens, 9 (8), p. 626 [22 p.].

Titre du document
New molecular data on Filaria and its Wolbachia from red howler monkeys (Alouatta macconnelli) in French Guiana : a preliminary study
Année de publication
2020
Type de document
Article référencé dans le Web of Science WOS:000567103900001
Auteurs
Laidoudi Y., Medkour H., Levasseur A., Davoust B., Mediannikov Oleg
Source
Pathogens, 2020, 9 (8), p. 626 [22 p.]
Previous studies have reported filarial parasites of the genus Dipetalonema and Mansonella from French Guiana monkeys, based on morphological taxonomy. In this study, we screened blood samples from nine howler monkeys (Alouatta macconnelli) for the presence of filaria and Wolbachia DNA. The infection rates were 88.9% for filaria and 55.6% for wolbachiae. The molecular characterization, based on the 18S gene of filariids, revealed that A. macconnelli are infected with at least three species (Mansonella sp., Brugia sp. and an unidentified Onchocercidae species.). Since the 18S and cox1 generic primers are not very effective at resolving co-infections, we developed ITS genus-specific PCRs for Mansonella and Brugia genus. The results revealed coinfections in 75% of positives. The presence of Mansonella sp. and Brugia sp. was also confirmed by the 16S phylogenetic analysis of their associated Wolbachia. Mansonella sp., which close to the species from the subgenus Tetrapetalonema encountered in NewWorld Monkeys, while Brugia sp. was identical to the strain circulating in French Guiana dogs. We propose a novel ITS1 Brugia genus-specific qPCR. We applied it to screen for Brugia infection in howler monkeys and 66.7% were found to be positive. Our finding highlights the need for further studies to clarify the species diversity of neotropics monkeys by combining molecular and morphological features. The novel Brugia genus-specific qPCR assays could be an effective tool for the surveillance and characterization of this potential zoonosis.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052] ; Sciences du monde animal [080]
Description Géographique
GUYANE FRANCAISE
Localisation
Fonds IRD [F B010079714]
Identifiant IRD
fdi:010079714
Contact