@article{fdi:010079714,
  title = {{N}ew molecular data on {F}ilaria and its {W}olbachia from red howler monkeys ({A}louatta macconnelli) in {F}rench {G}uiana : a preliminary study},
  author = {{L}aidoudi, {Y}. and {M}edkour, {H}. and {L}evasseur, {A}. and {D}avoust, {B}. and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{P}revious studies have reported filarial parasites of the genus {D}ipetalonema and {M}ansonella from {F}rench {G}uiana monkeys, based on morphological taxonomy. {I}n this study, we screened blood samples from nine howler monkeys ({A}louatta macconnelli) for the presence of filaria and {W}olbachia {DNA}. {T}he infection rates were 88.9% for filaria and 55.6% for wolbachiae. {T}he molecular characterization, based on the 18{S} gene of filariids, revealed that {A}. macconnelli are infected with at least three species ({M}ansonella sp., {B}rugia sp. and an unidentified {O}nchocercidae species.). {S}ince the 18{S} and cox1 generic primers are not very effective at resolving co-infections, we developed {ITS} genus-specific {PCR}s for {M}ansonella and {B}rugia genus. {T}he results revealed coinfections in 75% of positives. {T}he presence of {M}ansonella sp. and {B}rugia sp. was also confirmed by the 16{S} phylogenetic analysis of their associated {W}olbachia. {M}ansonella sp., which close to the species from the subgenus {T}etrapetalonema encountered in {N}ew{W}orld {M}onkeys, while {B}rugia sp. was identical to the strain circulating in {F}rench {G}uiana dogs. {W}e propose a novel {ITS}1 {B}rugia genus-specific q{PCR}. {W}e applied it to screen for {B}rugia infection in howler monkeys and 66.7% were found to be positive. {O}ur finding highlights the need for further studies to clarify the species diversity of neotropics monkeys by combining molecular and morphological features. {T}he novel {B}rugia genus-specific q{PCR} assays could be an effective tool for the surveillance and characterization of this potential zoonosis.},
  keywords = {{M}ansonella sp. ; {B}rugia sp. ; {O}nchocercidae sp. ; {W}olbachia ; neotropic ; monkeys ; reservoir ; zoonosis ; {GUYANE} {FRANCAISE}},
  booktitle = {},
  journal = {{P}athogens},
  volume = {9},
  numero = {8},
  pages = {626 [22 p.]},
  year = {2020},
  DOI = {10.3390/pathogens9080626},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079714},
}