Pastorino B., Touret F., Gilles Magali, Lamballerie X. de, Charrel R. N. (2020). Heat inactivation of different types of SARS-CoV-2 samples : what protocols for biosafety, molecular detection and serological diagnostics ?. Viruses, 12 (7), art. 735 [8 p.].
Titre du document
Heat inactivation of different types of SARS-CoV-2 samples : what protocols for biosafety, molecular detection and serological diagnostics ?
Pastorino B., Touret F., Gilles Magali, Lamballerie X. de, Charrel R. N.
Source
Viruses, 2020,
12 (7), art. 735 [8 p.]
Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. We evaluated three heat inactivation protocols (56 degrees C-30 min, 60 degrees C-60 min and 92 degrees C-15 min) on SARS-CoV-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the European norm NF EN 14476-A2. Regardless of the protocol and the type of samples, a 4 Log(10)TCID50 reduction was observed. However, samples containing viral loads > 6 Log(10)TCID(50)were still infectious after 56 degrees C-30 min and 60 degrees C-60 min, although infectivity was < 10 TCID50. The protocols 56 degrees C-30 min and 60 degrees C-60 min had little influence on the RNA copies detection, whereas 92 degrees C-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. Lastly, 56 degrees C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test, whereas a drastic decrease in neutralizing titers was observed.
