%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Pastorino, B.
%A Touret, F.
%A Gilles, Magali
%A Lamballerie, X. de
%A Charrel, R. N.
%T Heat inactivation of different types of SARS-CoV-2 samples : what protocols for biosafety, molecular detection and serological diagnostics ?
%D 2020
%L fdi:010079492
%G ENG
%J Viruses
%K SARS-CoV-2 ; coronavirus ; heat inactivation ; COVID-19 ; serology ; ELISA ; neutralization ; virus neutralization test
%M ISI:000557050000001
%N 7
%P art. 735 [8 ]
%R 10.3390/v12070735
%U https://www.documentation.ird.fr/hor/fdi:010079492
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-08/010079492.pdf
%V 12
%W Horizon (IRD)
%X Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. We evaluated three heat inactivation protocols (56 degrees C-30 min, 60 degrees C-60 min and 92 degrees C-15 min) on SARS-CoV-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the European norm NF EN 14476-A2. Regardless of the protocol and the type of samples, a 4 Log(10)TCID50 reduction was observed. However, samples containing viral loads > 6 Log(10)TCID(50)were still infectious after 56 degrees C-30 min and 60 degrees C-60 min, although infectivity was < 10 TCID50. The protocols 56 degrees C-30 min and 60 degrees C-60 min had little influence on the RNA copies detection, whereas 92 degrees C-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. Lastly, 56 degrees C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test, whereas a drastic decrease in neutralizing titers was observed.
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