@article{fdi:010079492,
  title = {{H}eat inactivation of different types of {SARS}-{C}o{V}-2 samples : what protocols for biosafety, molecular detection and serological diagnostics ?},
  author = {{P}astorino, {B}. and {T}ouret, {F}. and {G}illes, {M}agali and {L}amballerie, {X}. de and {C}harrel, {R}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}tandard precautions to minimize the risk of {SARS}-{C}o{V}-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. {W}e evaluated three heat inactivation protocols (56 degrees {C}-30 min, 60 degrees {C}-60 min and 92 degrees {C}-15 min) on {SARS}-{C}o{V}-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the {E}uropean norm {NF} {EN} 14476-{A}2. {R}egardless of the protocol and the type of samples, a 4 {L}og(10){TCID}50 reduction was observed. {H}owever, samples containing viral loads > 6 {L}og(10){TCID}(50)were still infectious after 56 degrees {C}-30 min and 60 degrees {C}-60 min, although infectivity was < 10 {TCID}50. {T}he protocols 56 degrees {C}-30 min and 60 degrees {C}-60 min had little influence on the {RNA} copies detection, whereas 92 degrees {C}-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. {L}astly, 56 degrees {C}-30 min treatment of serum specimens had a negligible influence on the results of {I}g{G} detection using a commercial {ELISA} test, whereas a drastic decrease in neutralizing titers was observed.},
  keywords = {{SARS}-{C}o{V}-2 ; coronavirus ; heat inactivation ; {COVID}-19 ; serology ; {ELISA} ; neutralization ; virus neutralization test},
  booktitle = {},
  journal = {{V}iruses},
  volume = {12},
  numero = {7},
  pages = {art. 735 [8 ]},
  year = {2020},
  DOI = {10.3390/v12070735},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079492},
}