Pezzi L., Charrel R. N., Ninove L., Nougairede A., Molle G., Coutard B., Durand G., Leparc-Goffart I., Lamballerie X. de, Thirion Laurence. (2020). Development and evaluation of a duo SARS-CoV-2 RT-qPCR assay combining two assays approved by the World Health Organization targeting the envelope and the RNA-Dependant RNA polymerase (RdRp) coding regions. Viruses, 12 (6), art. 686 [12p.].
Titre du document
Development and evaluation of a duo SARS-CoV-2 RT-qPCR assay combining two assays approved by the World Health Organization targeting the envelope and the RNA-Dependant RNA polymerase (RdRp) coding regions
Année de publication
2020
Auteurs
Pezzi L., Charrel R. N., Ninove L., Nougairede A., Molle G., Coutard B., Durand G., Leparc-Goffart I., Lamballerie X. de, Thirion Laurence
Source
Viruses, 2020,
12 (6), art. 686 [12p.]
The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has highlighted the importance of reliable and rapid diagnostic testing to prevent and control virus circulation. Dozens of monoplex in-house RT-qPCR assays are already available; however, the development of dual-target assays is suited to avoid false-negative results caused by polymorphisms or point mutations, that can compromise the accuracy of diagnostic and screening tests. In this study, two mono-target assays recommended by WHO (E-Sarbeco (enveloppe gene, Charite University, Berlin, Germany) and RdRp-IP4 (RdRp, Institut Pasteur, Paris, France)) were selected and combined in a unique robust test; the resulting duo SARS-CoV-2 RT-qPCR assay was compared to the two parental monoplex tests. The duo SARS-CoV-2 assay performed equally, or better, in terms of sensitivity, specificity, linearity and signal intensity. We demonstrated that combining two single systems into a dual-target assay (with or without an MS2-based internal control) did not impair performances, providing a potent tool adapted for routine molecular diagnosis in clinical microbiology laboratories.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010079428]
Identifiant IRD
fdi:010079428
