%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Pezzi, L.
%A Charrel, R. N.
%A Ninove, L.
%A Nougairede, A.
%A Molle, G.
%A Coutard, B.
%A Durand, G.
%A Leparc-Goffart, I.
%A Lamballerie, X. de
%A Thirion, Laurence
%T Development and evaluation of a duo SARS-CoV-2 RT-qPCR assay combining two assays approved by the World Health Organization targeting the envelope and the RNA-Dependant RNA polymerase (RdRp) coding regions
%D 2020
%L fdi:010079428
%G ENG
%J Viruses
%K coronavirus ; COVID-19 ; real-time PCR ; diagnostics ; diagnosis ; emerging ; respiratory ; molecular ; outbreak ; preparedness ; response ; TaqMan
%M ISI:000551247800001
%N 6
%P art. 686 [12]
%R 10.3390/v12060686
%U https://www.documentation.ird.fr/hor/fdi:010079428
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-08/010079428.pdf
%V 12
%W Horizon (IRD)
%X The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has highlighted the importance of reliable and rapid diagnostic testing to prevent and control virus circulation. Dozens of monoplex in-house RT-qPCR assays are already available; however, the development of dual-target assays is suited to avoid false-negative results caused by polymorphisms or point mutations, that can compromise the accuracy of diagnostic and screening tests. In this study, two mono-target assays recommended by WHO (E-Sarbeco (enveloppe gene, Charite University, Berlin, Germany) and RdRp-IP4 (RdRp, Institut Pasteur, Paris, France)) were selected and combined in a unique robust test; the resulting duo SARS-CoV-2 RT-qPCR assay was compared to the two parental monoplex tests. The duo SARS-CoV-2 assay performed equally, or better, in terms of sensitivity, specificity, linearity and signal intensity. We demonstrated that combining two single systems into a dual-target assay (with or without an MS2-based internal control) did not impair performances, providing a potent tool adapted for routine molecular diagnosis in clinical microbiology laboratories.
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