@article{fdi:010079428,
  title = {{D}evelopment and evaluation of a duo {SARS}-{C}o{V}-2 {RT}-q{PCR} assay combining two assays approved by the {W}orld {H}ealth {O}rganization targeting the envelope and the {RNA}-{D}ependant {RNA} polymerase ({R}d{R}p) coding regions},
  author = {{P}ezzi, {L}. and {C}harrel, {R}. {N}. and {N}inove, {L}. and {N}ougairede, {A}. and {M}olle, {G}. and {C}outard, {B}. and {D}urand, {G}. and {L}eparc-{G}offart, {I}. and {L}amballerie, {X}. de and {T}hirion, {L}aurence},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he recent emergence of severe acute respiratory syndrome coronavirus 2 ({SARS}-{C}o{V}-2) worldwide has highlighted the importance of reliable and rapid diagnostic testing to prevent and control virus circulation. {D}ozens of monoplex in-house {RT}-q{PCR} assays are already available; however, the development of dual-target assays is suited to avoid false-negative results caused by polymorphisms or point mutations, that can compromise the accuracy of diagnostic and screening tests. {I}n this study, two mono-target assays recommended by {WHO} ({E}-{S}arbeco (enveloppe gene, {C}harite {U}niversity, {B}erlin, {G}ermany) and {R}d{R}p-{IP}4 ({R}d{R}p, {I}nstitut {P}asteur, {P}aris, {F}rance)) were selected and combined in a unique robust test; the resulting duo {SARS}-{C}o{V}-2 {RT}-q{PCR} assay was compared to the two parental monoplex tests. {T}he duo {SARS}-{C}o{V}-2 assay performed equally, or better, in terms of sensitivity, specificity, linearity and signal intensity. {W}e demonstrated that combining two single systems into a dual-target assay (with or without an {MS}2-based internal control) did not impair performances, providing a potent tool adapted for routine molecular diagnosis in clinical microbiology laboratories.},
  keywords = {coronavirus ; {COVID}-19 ; real-time {PCR} ; diagnostics ; diagnosis ; emerging ; respiratory ; molecular ; outbreak ; preparedness ; response ; {T}aq{M}an},
  booktitle = {},
  journal = {{V}iruses},
  volume = {12},
  numero = {6},
  pages = {art. 686 [12]},
  year = {2020},
  DOI = {10.3390/v12060686},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079428},
}