Publications des scientifiques de l'IRD

Flores C., Zarate C., Triplett L., Maillot-Lebon V., Moufid Y., Kante M., Bragard C., Verdier Valérie, Gagnevin L., Szurek Boris, Robene I. (2019). Development of a duplex-PCR for differential diagnosis of Xanthomonas phaseoli pv. manihotis and Xanthomonas cassavae in cassava (Manihot esculenta). Physiological and Molecular Plant Pathology, 105 (SI), p. 34-46. ISSN 0885-5765.

Titre du document
Development of a duplex-PCR for differential diagnosis of Xanthomonas phaseoli pv. manihotis and Xanthomonas cassavae in cassava (Manihot esculenta)
Année de publication
2019
Type de document
Article référencé dans le Web of Science WOS:000457299200005
Auteurs
Flores C., Zarate C., Triplett L., Maillot-Lebon V., Moufid Y., Kante M., Bragard C., Verdier Valérie, Gagnevin L., Szurek Boris, Robene I.
Source
Physiological and Molecular Plant Pathology, 2019, 105 (SI), p. 34-46 ISSN 0885-5765
Cassava Bacterial Blight and Cassava Bacterial Necrosis are two bacterial diseases affecting cassava, respectively caused by Xanthomonas phaseoli pv. manihotis (Xpm) and Xanthomonas cassavae (Xc). Since both pathogens may be present on leaves and cause similar symptoms, we developed a new molecular diagnostic tool to detect and distinguish between Xpm and Xc. Based on genome sequences from the target species as well as from non-target species, in silico analysis was performed to select candidate primers through a novel strategy targeting specificity. Experimental validation enabled to establish a duplex-PCR diagnostic tool that will be useful for future surveillance programs and better disease control.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010075151]
Identifiant IRD
fdi:010075151
Contact