Flores C. auteur aut IRD Zarate C. auteur aut IRD Triplett L. auteur aut IRD Maillot-Lebon V. auteur aut IRD Moufid Y. auteur aut IRD Kante M. auteur aut IRD Bragard C. auteur aut IRD Verdier Valérie auteur aut IRD Gagnevin L. auteur aut IRD Szurek Boris auteur aut IRD Robene I. auteur aut IRD text journalArticle eng text/pdf born digital access Cassava Bacterial Blight and Cassava Bacterial Necrosis are two bacterial diseases affecting cassava, respectively caused by Xanthomonas phaseoli pv. manihotis (Xpm) and Xanthomonas cassavae (Xc). Since both pathogens may be present on leaves and cause similar symptoms, we developed a new molecular diagnostic tool to detect and distinguish between Xpm and Xc. Based on genome sequences from the target species as well as from non-target species, in silico analysis was performed to select candidate primers through a novel strategy targeting specificity. Experimental validation enabled to establish a duplex-PCR diagnostic tool that will be useful for future surveillance programs and better disease control. specialized Cassava Diagnostic PCR Xanthomonas phaseoli pv. manihotis Xanthomonas cassavae 076 105 SI 34-46 2019 0885-5765 https://www.documentation.ird.fr/hor/fdi:010075151 10.1016/j.pmpp.2018.07.005 0885-5765 [F B010075151] https://www.documentation.ird.fr/hor/fdi:010075151 https://www.documentation.ird.fr/intranet/publi/2019/02/010075151.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-03-06 2025-02-24 fdi:010075151 fre