@article{fdi:010075151,
  title = {{D}evelopment of a duplex-{PCR} for differential diagnosis of {X}anthomonas phaseoli pv. manihotis and {X}anthomonas cassavae in cassava ({M}anihot esculenta)},
  author = {{F}lores, {C}. and {Z}arate, {C}. and {T}riplett, {L}. and {M}aillot-{L}ebon, {V}. and {M}oufid, {Y}. and {K}ante, {M}. and {B}ragard, {C}. and {V}erdier, {V}al{\'e}rie and {G}agnevin, {L}. and {S}zurek, {B}oris and {R}obene, {I}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}assava {B}acterial {B}light and {C}assava {B}acterial {N}ecrosis are two bacterial diseases affecting cassava, respectively caused by {X}anthomonas phaseoli pv. manihotis ({X}pm) and {X}anthomonas cassavae ({X}c). {S}ince both pathogens may be present on leaves and cause similar symptoms, we developed a new molecular diagnostic tool to detect and distinguish between {X}pm and {X}c. {B}ased on genome sequences from the target species as well as from non-target species, in silico analysis was performed to select candidate primers through a novel strategy targeting specificity. {E}xperimental validation enabled to establish a duplex-{PCR} diagnostic tool that will be useful for future surveillance programs and better disease control.},
  keywords = {{C}assava ; {D}iagnostic {PCR} ; {X}anthomonas phaseoli pv. manihotis ; {X}anthomonas cassavae},
  booktitle = {},
  journal = {{P}hysiological and {M}olecular {P}lant {P}athology},
  volume = {105},
  numero = {{SI}},
  pages = {34--46},
  ISSN = {0885-5765},
  year = {2019},
  DOI = {10.1016/j.pmpp.2018.07.005},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075151},
}