Publications des scientifiques de l'IRD

Hily J. M., Demaneche S., Poulicard Nils, Tannieres M., Djennane S., Beuve M., Vigne E., Demangeat G., Komar V., Gertz C., Marmonier A., Hemmer C., Vigneron S., Marais A., Candresse T., Simonet P., Lemaire O. (2018). Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome. Plant Biotechnology Journal, 16 (1), p. 208-220. ISSN 1467-7644.

Titre du document
Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome
Année de publication
2018
Type de document
Article référencé dans le Web of Science WOS:000423363300019
Auteurs
Hily J. M., Demaneche S., Poulicard Nils, Tannieres M., Djennane S., Beuve M., Vigne E., Demangeat G., Komar V., Gertz C., Marmonier A., Hemmer C., Vigneron S., Marais A., Candresse T., Simonet P., Lemaire O.
Source
Plant Biotechnology Journal, 2018, 16 (1), p. 208-220 ISSN 1467-7644
For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus-resistant transgenic plants based on the pathogen-derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified (GM) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus (GFLV) populations and nontargeted root-associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years.
Plan de classement
Biologie du sol [074] ; Sciences du monde végétal [076] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010072009]
Identifiant IRD
fdi:010072009
Contact