Hily J. M. auteur aut IRD Demaneche S. auteur aut IRD Poulicard Nils auteur aut IRD Tannieres M. auteur aut IRD Djennane S. auteur aut IRD Beuve M. auteur aut IRD Vigne E. auteur aut IRD Demangeat G. auteur aut IRD Komar V. auteur aut IRD Gertz C. auteur aut IRD Marmonier A. auteur aut IRD Hemmer C. auteur aut IRD Vigneron S. auteur aut IRD Marais A. auteur aut IRD Candresse T. auteur aut IRD Simonet P. auteur aut IRD Lemaire O. auteur aut IRD text journalArticle eng text/pdf reformatted digital access For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus-resistant transgenic plants based on the pathogen-derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified (GM) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus (GFLV) populations and nontargeted root-associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years. specialized environmental microbiology metagenomics grapevine transgenic rootstock safety and regulatory affairs 084 076 074 16 1 208-220 2018 1467-7644 https://www.documentation.ird.fr/hor/fdi:010072009 10.1111/pbi.12761 1467-7644 [F B010072009] https://www.documentation.ird.fr/hor/fdi:010072009 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-02/010072009.pdf IRD - Base Horizon / Pleins textes 2018-03-05 2023-07-11 fdi:010072009 fre