Triplett L. R., Verdier Valérie, Campillo T., Van Malderghem C., Cleenwerck I., Maes M., Deblais L., Corral R., Koita O., Cottyn B., Leach J. E. (2015). Characterization of a novel clade of Xanthomonas isolated from rice leaves in Mali and proposal of Xanthomonas maliensis sp. nov.. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 107 (4), p. 869-881. ISSN 0003-6072.
Titre du document
Characterization of a novel clade of Xanthomonas isolated from rice leaves in Mali and proposal of Xanthomonas maliensis sp. nov.
Triplett L. R., Verdier Valérie, Campillo T., Van Malderghem C., Cleenwerck I., Maes M., Deblais L., Corral R., Koita O., Cottyn B., Leach J. E.
Source
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2015,
107 (4), p. 869-881 ISSN 0003-6072
Four bacterial strains, designated M89, M92, M97(T), and M106, were isolated in a previous study from surface-sterilized leaves of rice (Oryza sativa) or murainagrass (Ischaemum rugosum) at three sites in Mali, Africa. Here they were examined by a polyphasic taxonomic approach and analysis of a whole-genome sequence. Phylogenetic analyses based on 16S rRNA sequence and multilocus sequence analysis of seven genes showed that these four strains formed a distinct lineage representing a novel species within the genus Xanthomonas. This was supported by whole-genome average nucleotide identity values calculated from comparisons of strain M97(T) with established Xanthomonas species. The strains can be differentiated from the known Xanthomonas species on the basis of their fatty acid and carbohydrate utilization profiles. Population growth studies on rice confirmed that these bacteria multiply in rice leaves without causing symptoms. Identification of this novel species can be accomplished by using diagnostic primer sets or by gyrB gene sequence analysis. We propose to classify these rice- and grass-associated bacteria as Xanthomonas maliensis sp. nov. with strain M97(T) = CFBP7942(T) = LMG27592(T) as the type strain.
Plan de classement
Sciences du monde végétal [076]
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Biotechnologies [084]
