Triplett L. R. auteur aut IRD Verdier Valérie auteur aut IRD Campillo T. auteur aut IRD Van Malderghem C. auteur aut IRD Cleenwerck I. auteur aut IRD Maes M. auteur aut IRD Deblais L. auteur aut IRD Corral R. auteur aut IRD Koita O. auteur aut IRD Cottyn B. auteur aut IRD Leach J. E. auteur aut IRD text journalArticle eng text/pdf born digital access Four bacterial strains, designated M89, M92, M97(T), and M106, were isolated in a previous study from surface-sterilized leaves of rice (Oryza sativa) or murainagrass (Ischaemum rugosum) at three sites in Mali, Africa. Here they were examined by a polyphasic taxonomic approach and analysis of a whole-genome sequence. Phylogenetic analyses based on 16S rRNA sequence and multilocus sequence analysis of seven genes showed that these four strains formed a distinct lineage representing a novel species within the genus Xanthomonas. This was supported by whole-genome average nucleotide identity values calculated from comparisons of strain M97(T) with established Xanthomonas species. The strains can be differentiated from the known Xanthomonas species on the basis of their fatty acid and carbohydrate utilization profiles. Population growth studies on rice confirmed that these bacteria multiply in rice leaves without causing symptoms. Identification of this novel species can be accomplished by using diagnostic primer sets or by gyrB gene sequence analysis. We propose to classify these rice- and grass-associated bacteria as Xanthomonas maliensis sp. nov. with strain M97(T) = CFBP7942(T) = LMG27592(T) as the type strain. specialized Xanthomonas MLSA Oryza sativa ANI MALI 076 084 107 4 869-881 2015 0003-6072 https://www.documentation.ird.fr/hor/fdi:010064023 10.1007/s10482-015-0379-5 0003-6072 [F B010064023] https://www.documentation.ird.fr/hor/fdi:010064023 https://www.documentation.ird.fr/intranet/publi/2015/04/010064023.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-05-04 2017-08-23 fdi:010064023 fre