Publications des scientifiques de l'IRD

Sérémé D., Lacombe Séverine, Konaté M., Bangratz Martine, Galzi Agnès, Fargette Denis, Traoré A. S., Konaté G., Brugidou Christophe. (2014). Sites under positive selection modulate the RNA silencing suppressor activity of rice yellow mottle virus movement protein P1. Journal of General Virology, 95 (1), p. 213-218. ISSN 0022-1317.

Titre du document
Sites under positive selection modulate the RNA silencing suppressor activity of rice yellow mottle virus movement protein P1
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000338177300023
Auteurs
Sérémé D., Lacombe Séverine, Konaté M., Bangratz Martine, Galzi Agnès, Fargette Denis, Traoré A. S., Konaté G., Brugidou Christophe
Source
Journal of General Virology, 2014, 95 (1), p. 213-218 ISSN 0022-1317
RNA silencing is a eukaryotic mechanism for RNA-based gene regulation that plays an essential role in diverse biological processes, such as defence against viral infections. The P1 of rice yellow mottle virus (RYMV) is a movement protein and displays RNA silencing suppression activity with variable efficiency, depending on the origin of the isolates. In this study, the positive selection pressure acting on the P1 protein gene was assessed. A site-by-site analysis of the d(N)/d(S) ratio was performed and 18 positively selected sites were identified. Four of these were mutated, and the ability to suppress RNA silencing was evaluated for the resulting mutants in a transient expression assay. All mutations affected quantitatively RNA silencing suppression, one caused a significant decrease in the activity and three significantly increased it. This work demonstrates, for what is to the best of our knowledge the first time, that the RYMV gene encoding the P1 RNA silencing suppressor is under adaptive evolution.
Plan de classement
Sciences du monde végétal [076] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010062392]
Identifiant IRD
fdi:010062392
Contact