Sérémé D. auteur aut IRD Lacombe Séverine auteur aut IRD Konaté M. auteur aut IRD Bangratz Martine auteur aut IRD Galzi Agnès auteur aut IRD Fargette Denis auteur aut IRD Traoré A. S. auteur aut IRD Konaté G. auteur aut IRD Brugidou Christophe auteur aut IRD text journalArticle eng text/pdf born digital access RNA silencing is a eukaryotic mechanism for RNA-based gene regulation that plays an essential role in diverse biological processes, such as defence against viral infections. The P1 of rice yellow mottle virus (RYMV) is a movement protein and displays RNA silencing suppression activity with variable efficiency, depending on the origin of the isolates. In this study, the positive selection pressure acting on the P1 protein gene was assessed. A site-by-site analysis of the d(N)/d(S) ratio was performed and 18 positively selected sites were identified. Four of these were mutated, and the ability to suppress RNA silencing was evaluated for the resulting mutants in a transient expression assay. All mutations affected quantitatively RNA silencing suppression, one caused a significant decrease in the activity and three significantly increased it. This work demonstrates, for what is to the best of our knowledge the first time, that the RYMV gene encoding the P1 RNA silencing suppressor is under adaptive evolution. specialized 076 084 95 1 213-218 2014 0022-1317 https://www.documentation.ird.fr/hor/fdi:010062392 10.1099/vir.0.057026-0 0022-1317 [F B010062392] https://www.documentation.ird.fr/hor/fdi:010062392 https://www.documentation.ird.fr/intranet/publi/2014/08/010062392.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-09-09 2017-08-23 fdi:010062392 fre