@article{fdi:010062392,
  title = {{S}ites under positive selection modulate the {RNA} silencing suppressor activity of rice yellow mottle virus movement protein {P}1},
  author = {{S}{\'e}r{\'e}m{\'e}, {D}. and {L}acombe, {S}{\'e}verine and {K}onat{\'e}, {M}. and {B}angratz, {M}artine and {G}alzi, {A}gn{\`e}s and {F}argette, {D}enis and {T}raor{\'e}, {A}. {S}. and {K}onat{\'e}, {G}. and {B}rugidou, {C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{RNA} silencing is a eukaryotic mechanism for {RNA}-based gene regulation that plays an essential role in diverse biological processes, such as defence against viral infections. {T}he {P}1 of rice yellow mottle virus ({RYMV}) is a movement protein and displays {RNA} silencing suppression activity with variable efficiency, depending on the origin of the isolates. {I}n this study, the positive selection pressure acting on the {P}1 protein gene was assessed. {A} site-by-site analysis of the d({N})/d({S}) ratio was performed and 18 positively selected sites were identified. {F}our of these were mutated, and the ability to suppress {RNA} silencing was evaluated for the resulting mutants in a transient expression assay. {A}ll mutations affected quantitatively {RNA} silencing suppression, one caused a significant decrease in the activity and three significantly increased it. {T}his work demonstrates, for what is to the best of our knowledge the first time, that the {RYMV} gene encoding the {P}1 {RNA} silencing suppressor is under adaptive evolution.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {G}eneral {V}irology},
  volume = {95},
  numero = {1},
  pages = {213--218},
  ISSN = {0022-1317},
  year = {2014},
  DOI = {10.1099/vir.0.057026-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062392},
}