Talhinhas P., Azinheira H. G., Vieira B., Loureiro A., Tavares S., Batista D., Morin E., Petitot Anne-Sophie, Paulo O. S., Poulain J., Da Silva C., Duplessis S., Silva M. D., Fernandez Diana. (2014). Overview of the functional virulent genome of the coffee leaf rust pathogen Hemileia vastatrix with an emphasis on early stages of infection. Frontiers in Plant Science, 5, art. 88 [17 p.]. ISSN 1664-462X.
Titre du document
Overview of the functional virulent genome of the coffee leaf rust pathogen Hemileia vastatrix with an emphasis on early stages of infection
Année de publication
2014
Auteurs
Talhinhas P., Azinheira H. G., Vieira B., Loureiro A., Tavares S., Batista D., Morin E., Petitot Anne-Sophie, Paulo O. S., Poulain J., Da Silva C., Duplessis S., Silva M. D., Fernandez Diana
Source
Frontiers in Plant Science, 2014,
5, art. 88 [17 p.] ISSN 1664-462X
Hemileia vastatrix is the causal agent of coffee leaf rust, the most important disease of coffee Arabica. In this work, a 454-pyrosequencing transcriptome analysis of H. vastatrix germinating urediniospores (gU) and appressoria (Ap) was performed and compared to previously published in planta haustoria-rich (H) data. A total of 9234 transcripts were identified and annotated. Ca. 50% of these transcripts showed no significant homology to international databases. Only 784 sequences were shared by the three conditions, and 75% were exclusive of either gU (2146), Ap (1479) or H (3270). Relative transcript abundance and RT-qPCR analyses for a selection of genes indicated a particularly active metabolism, translational activity and production of new structures in the appressoria and intense signaling, transport, secretory activity and cellular multiplication in the germinating urediniospores, suggesting the onset of a plant-fungus dialogue as early as at the germ tube stage. Gene expression related to the production of carbohydrate-active enzymes and accumulation of glycerol in germinating urediniospores and appressoria suggests that combined lytic and physical mechanisms are involved in appressoria-mediated penetration. Besides contributing to the characterization of molecular processes leading to appressoria-mediated infection by rust fungi, these results point toward the identification of new H. vastatrix candidate virulence factors, with 516 genes predicted to encode secreted proteins.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010061842]
Identifiant IRD
fdi:010061842
