@article{fdi:010061842,
  title = {{O}verview of the functional virulent genome of the coffee leaf rust pathogen {H}emileia vastatrix with an emphasis on early stages of infection},
  author = {{T}alhinhas, {P}. and {A}zinheira, {H}. {G}. and {V}ieira, {B}. and {L}oureiro, {A}. and {T}avares, {S}. and {B}atista, {D}. and {M}orin, {E}. and {P}etitot, {A}nne-{S}ophie and {P}aulo, {O}. {S}. and {P}oulain, {J}. and {D}a {S}ilva, {C}. and {D}uplessis, {S}. and {S}ilva, {M}. {D}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{H}emileia vastatrix is the causal agent of coffee leaf rust, the most important disease of coffee {A}rabica. {I}n this work, a 454-pyrosequencing transcriptome analysis of {H}. vastatrix germinating urediniospores (g{U}) and appressoria ({A}p) was performed and compared to previously published in planta haustoria-rich ({H}) data. {A} total of 9234 transcripts were identified and annotated. {C}a. 50% of these transcripts showed no significant homology to international databases. {O}nly 784 sequences were shared by the three conditions, and 75% were exclusive of either g{U} (2146), {A}p (1479) or {H} (3270). {R}elative transcript abundance and {RT}-q{PCR} analyses for a selection of genes indicated a particularly active metabolism, translational activity and production of new structures in the appressoria and intense signaling, transport, secretory activity and cellular multiplication in the germinating urediniospores, suggesting the onset of a plant-fungus dialogue as early as at the germ tube stage. {G}ene expression related to the production of carbohydrate-active enzymes and accumulation of glycerol in germinating urediniospores and appressoria suggests that combined lytic and physical mechanisms are involved in appressoria-mediated penetration. {B}esides contributing to the characterization of molecular processes leading to appressoria-mediated infection by rust fungi, these results point toward the identification of new {H}. vastatrix candidate virulence factors, with 516 genes predicted to encode secreted proteins.},
  keywords = {appressorium ; coffee leaf rust ; germinating urediniospore ; haustorium ; pyrosequencing ; transcriptome},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {5},
  numero = {},
  pages = {art. 88 [17 ]},
  ISSN = {1664-462{X}},
  year = {2014},
  DOI = {10.3389/fpls.2014.00088},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061842},
}