Overview of the functional virulent genome of the coffee leaf rust pathogen Hemileia vastatrix with an emphasis on early stages of infection

Overview of the functional virulent genome of the coffee leaf rust pathogen Hemileia vastatrix with an emphasis on early stages of infection Talhinhas P. auteur aut IRD Azinheira H. G. auteur aut IRD Vieira B. auteur aut IRD Loureiro A. auteur aut IRD Tavares S. auteur aut IRD Batista D. auteur aut IRD Morin E. auteur aut IRD Petitot Anne-Sophie auteur aut IRD Paulo O. S. auteur aut IRD Poulain J. auteur aut IRD Da Silva C. auteur aut IRD Duplessis S. auteur aut IRD Silva M. D. auteur aut IRD Fernandez Diana auteur aut IRD text journalArticle eng text/pdf born digital access Hemileia vastatrix is the causal agent of coffee leaf rust, the most important disease of coffee Arabica. In this work, a 454-pyrosequencing transcriptome analysis of H. vastatrix germinating urediniospores (gU) and appressoria (Ap) was performed and compared to previously published in planta haustoria-rich (H) data. A total of 9234 transcripts were identified and annotated. Ca. 50% of these transcripts showed no significant homology to international databases. Only 784 sequences were shared by the three conditions, and 75% were exclusive of either gU (2146), Ap (1479) or H (3270). Relative transcript abundance and RT-qPCR analyses for a selection of genes indicated a particularly active metabolism, translational activity and production of new structures in the appressoria and intense signaling, transport, secretory activity and cellular multiplication in the germinating urediniospores, suggesting the onset of a plant-fungus dialogue as early as at the germ tube stage. Gene expression related to the production of carbohydrate-active enzymes and accumulation of glycerol in germinating urediniospores and appressoria suggests that combined lytic and physical mechanisms are involved in appressoria-mediated penetration. Besides contributing to the characterization of molecular processes leading to appressoria-mediated infection by rust fungi, these results point toward the identification of new H. vastatrix candidate virulence factors, with 516 genes predicted to encode secreted proteins. specialized appressorium coffee leaf rust germinating urediniospore haustorium pyrosequencing transcriptome 076 Frontiers in Plant Science 5 art. 88 [17 ] 2014 1664-462X https://www.documentation.ird.fr/hor/fdi:010061842 10.3389/fpls.2014.00088 1664-462X [F B010061842] https://www.documentation.ird.fr/hor/fdi:010061842 https://www.documentation.ird.fr/intranet/publi/2014/04/010061842.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-04-29 2017-08-23 fdi:010061842 fre