Ozudogru E. A., Kirdok E., Kaya E., Capuana M., Benelli C., Engelmann Florent. (2011). Cryopreservation of redwood (Sequoia sempervirens (D. DON.) ENDL.) in vitro buds using vitrification-based techniques. Cryoletters, 32 (2), p. 99-110. ISSN 0143-2044.
Titre du document
Cryopreservation of redwood (Sequoia sempervirens (D. DON.) ENDL.) in vitro buds using vitrification-based techniques
Ozudogru E. A., Kirdok E., Kaya E., Capuana M., Benelli C., Engelmann Florent
Source
Cryoletters, 2011,
32 (2), p. 99-110 ISSN 0143-2044
In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation-vitrification and droplet-vitrification were compared for cryopreserving Sequoia sempervirens apical and basal buds sampled from in vitro shoot cultures. The effect of cold-hardening of mother-plants and of bud culture medium and sucrose preculture was also investigated. Culture of apical and basal buds sampled from cold-hardened mother-plants on Quoirin and Lepoivre medium with activated charcoal had a positive effect on regrowth. Only droplet-vitrification ensured survival and regrowth after cryopreservation. After cryopreservation, regeneration of apical buds was possible for PVS2 exposure durations between 90 and 180 min but it remained low, with a maximum of 18% after 135 min treatment. With basal buds, regeneration after cryopreservation was possible over a larger range of PVS2 treatment durations, between 30 and 180 min. The highest regeneration percentage was slightly higher (22%) than that measured with apical buds, and was also achieved after 135 min PVS2 exposure.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
