%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Ozudogru, E. A.
%A Kirdok, E.
%A Kaya, E.
%A Capuana, M.
%A Benelli, C.
%A Engelmann, Florent
%T Cryopreservation of redwood (Sequoia sempervirens (D. DON.) ENDL.) in vitro buds using vitrification-based techniques
%D 2011
%L fdi:010053553
%G ENG
%J Cryoletters
%@ 0143-2044
%K conservation ; genetic resources ; redwood ; cryopreservation ; droplet-vitrification
%M ISI:000290888700002
%N 2
%P 99-110
%U https://www.documentation.ird.fr/hor/fdi:010053553
%> https://www.documentation.ird.fr/intranet/publi/2011/06/010053553.pdf
%V 32
%W Horizon (IRD)
%X In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation-vitrification and droplet-vitrification were compared for cryopreserving Sequoia sempervirens apical and basal buds sampled from in vitro shoot cultures. The effect of cold-hardening of mother-plants and of bud culture medium and sucrose preculture was also investigated. Culture of apical and basal buds sampled from cold-hardened mother-plants on Quoirin and Lepoivre medium with activated charcoal had a positive effect on regrowth. Only droplet-vitrification ensured survival and regrowth after cryopreservation. After cryopreservation, regeneration of apical buds was possible for PVS2 exposure durations between 90 and 180 min but it remained low, with a maximum of 18% after 135 min treatment. With basal buds, regeneration after cryopreservation was possible over a larger range of PVS2 treatment durations, between 30 and 180 min. The highest regeneration percentage was slightly higher (22%) than that measured with apical buds, and was also achieved after 135 min PVS2 exposure.
%$ 076 ; 084