@article{fdi:010053553,
  title = {{C}ryopreservation of redwood ({S}equoia sempervirens ({D}. {DON}.) {ENDL}.) in vitro buds using vitrification-based techniques},
  author = {{O}zudogru, {E}. {A}. and {K}irdok, {E}. and {K}aya, {E}. and {C}apuana, {M}. and {B}enelli, {C}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation-vitrification and droplet-vitrification were compared for cryopreserving {S}equoia sempervirens apical and basal buds sampled from in vitro shoot cultures. {T}he effect of cold-hardening of mother-plants and of bud culture medium and sucrose preculture was also investigated. {C}ulture of apical and basal buds sampled from cold-hardened mother-plants on {Q}uoirin and {L}epoivre medium with activated charcoal had a positive effect on regrowth. {O}nly droplet-vitrification ensured survival and regrowth after cryopreservation. {A}fter cryopreservation, regeneration of apical buds was possible for {PVS}2 exposure durations between 90 and 180 min but it remained low, with a maximum of 18% after 135 min treatment. {W}ith basal buds, regeneration after cryopreservation was possible over a larger range of {PVS}2 treatment durations, between 30 and 180 min. {T}he highest regeneration percentage was slightly higher (22%) than that measured with apical buds, and was also achieved after 135 min {PVS}2 exposure.},
  keywords = {conservation ; genetic resources ; redwood ; cryopreservation ; droplet-vitrification},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {32},
  numero = {2},
  pages = {99--110},
  ISSN = {0143-2044},
  year = {2011},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053553},
}