Esnault C., Boulesteix M., Duchemin J.B., Koffi A.A., Chandre Fabrice, Dabiré R., Robert Vincent, Simard Frédéric, Tripet F., Donnelly M.J., Fontenille Didier, Biémont C. (2008). High genetic differentiation between the M and S molecular forms of Anopheles gambiae in Africa. PLoS One, 3 (4), p. e1968. ISSN 1932-6203.
Titre du document
High genetic differentiation between the M and S molecular forms of Anopheles gambiae in Africa
Année de publication
2008
Auteurs
Esnault C., Boulesteix M., Duchemin J.B., Koffi A.A., Chandre Fabrice, Dabiré R., Robert Vincent, Simard Frédéric, Tripet F., Donnelly M.J., Fontenille Didier, Biémont C.
Source
PLoS One, 2008,
3 (4), p. e1968 ISSN 1932-6203
Background. Anopheles gambiae, a major vector of malaria, is widely distributed throughout sub-Saharan Africa. In an attempt to eliminate infective mosquitoes, researchers are trying to develop transgenic strains that are refractory to the Plasmodium parasite. Before any release of transgenic mosquitoes can be envisaged, we need an accurate picture of the differentiation between the two molecular forms of An. gambiae, termed M and S, which are of uncertain taxonomic status. Methodology/Principal Findings. Insertion patterns of three transposable elements (TEs) were determined in populations from Benin, Burkina Faso, Cameroon, Ghana, Ivory Coast, Madagascar, Mali, Mozambique, Niger, and Tanzania, using Transposon Display, a TE-anchored strategy based on Amplified Fragment Length Polymorphism. The results reveal a clear differentiation between the M and S forms, whatever their geographical origin, suggesting an incipient speciation process. Conclusions/Significance. Any attempt to control the transmission of malaria by An. gambiae using either conventional or novel technologies must take the M/S genetic differentiation into account. In addition, we localized three TE insertion sites that were present either in every individual or at a high frequency in the M molecular form. These sites were found to be located outside the chromosomal regions that are suspected of involvement in the speciation event between the two forms. This suggests that these chromosomal regions are either larger than previously thought, or there are additional differentiated genomic regions interspersed with undifferentiated regions.
Plan de classement
Anophèles et paludisme [052ANOPAL]
Descripteurs
PALUDISME ; VECTEUR ; MOUSTIQUE ; POLYMORPHISME INTRASPECIFIQUE ; SPECIATION ; STRUCTURE GENETIQUE ; ANALYSE GENETIQUE ; TRANSPOSON ; TECHNIQUE RFLP ; FLUX DE GENES
Description Géographique
AFRIQUE
Localisation
Fonds IRD [F B010050152]
Identifiant IRD
fdi:010050152
