Laurent T., Van der Auwera G., Hide Mallorie, Mertens P., Quispe Tintaya W., Deborggraeve S., De Doncker S., Lectipteux T., Banuls Anne-Laure, Buscher P., Dujardin J. C. (2009). Identification of Old World Leishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase B genes and rapid. Diagnostic Microbiology and Infectious Disease, 63 (2), p. 173-181. ISSN 0732-8893.
Titre du document
Identification of Old World Leishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase B genes and rapid
Année de publication
2009
Auteurs
Laurent T., Van der Auwera G., Hide Mallorie, Mertens P., Quispe Tintaya W., Deborggraeve S., De Doncker S., Lectipteux T., Banuls Anne-Laure, Buscher P., Dujardin J. C.
Source
Diagnostic Microbiology and Infectious Disease, 2009,
63 (2), p. 173-181 ISSN 0732-8893
We used the cysteine proteinase B (cpb) gene family of the trypanosomatid genus Leishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction (PCR) tests to discriminate Leishmania infantum, Leishmania donovani, Leishmania tropica, Leishmania aethiopica, and Leishmania major. Identification of all 5 Old World species and validation of intraspecies variability are features lacking in other species-specific PCRs. Amplicon analysis was done oil agarose gels and was further simplified by using an oligochrorriatography dipstick to detect L. infantum and L. donovani products. Because the analytical sensitivity is lower than that of certain other species- and genus-specific PCRs, our assays are especially valuable for use oil cultured isolates or directly on cryostabilates. As such, they can be implemented by research and health centers having access to Culturing, DNA isolation, and PCR.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010044248]
Identifiant IRD
fdi:010044248
