Laurent T. auteur aut IRD Van der Auwera G. auteur aut IRD Hide Mallorie auteur aut IRD Mertens P. auteur aut IRD Quispe Tintaya W. auteur aut IRD Deborggraeve S. auteur aut IRD De Doncker S. auteur aut IRD Lectipteux T. auteur aut IRD Banuls Anne-Laure auteur aut IRD Buscher P. auteur aut IRD Dujardin J. C. auteur aut IRD text journalArticle eng text/pdf born digital access We used the cysteine proteinase B (cpb) gene family of the trypanosomatid genus Leishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction (PCR) tests to discriminate Leishmania infantum, Leishmania donovani, Leishmania tropica, Leishmania aethiopica, and Leishmania major. Identification of all 5 Old World species and validation of intraspecies variability are features lacking in other species-specific PCRs. Amplicon analysis was done oil agarose gels and was further simplified by using an oligochrorriatography dipstick to detect L. infantum and L. donovani products. Because the analytical sensitivity is lower than that of certain other species- and genus-specific PCRs, our assays are especially valuable for use oil cultured isolates or directly on cryostabilates. As such, they can be implemented by research and health centers having access to Culturing, DNA isolation, and PCR. specialized Cysteine protease B Leishmaniasis Trypanosomatidae Oligochromatography Dipstick Differential diagnosis 052 63 2 173-181 2009 0732-8893 https://www.documentation.ird.fr/hor/fdi:010044248 10.1016/j.diagmicrobio.2008.10.015 0732-8893 [F B010044248] https://www.documentation.ird.fr/hor/fdi:010044248 https://www.documentation.ird.fr/intranet/publi/2009/02/010044248.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-03-06 2017-08-23 fdi:010044248 fre