@article{fdi:010044248,
  title = {{I}dentification of {O}ld {W}orld {L}eishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase {B} genes and rapid},
  author = {{L}aurent, {T}. and {V}an der {A}uwera, {G}. and {H}ide, {M}allorie and {M}ertens, {P}. and {Q}uispe {T}intaya, {W}. and {D}eborggraeve, {S}. and {D}e {D}oncker, {S}. and {L}ectipteux, {T}. and {B}anuls, {A}nne-{L}aure and {B}uscher, {P}. and {D}ujardin, {J}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e used the cysteine proteinase {B} (cpb) gene family of the trypanosomatid genus {L}eishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction ({PCR}) tests to discriminate {L}eishmania infantum, {L}eishmania donovani, {L}eishmania tropica, {L}eishmania aethiopica, and {L}eishmania major. {I}dentification of all 5 {O}ld {W}orld species and validation of intraspecies variability are features lacking in other species-specific {PCR}s. {A}mplicon analysis was done oil agarose gels and was further simplified by using an oligochrorriatography dipstick to detect {L}. infantum and {L}. donovani products. {B}ecause the analytical sensitivity is lower than that of certain other species- and genus-specific {PCR}s, our assays are especially valuable for use oil cultured isolates or directly on cryostabilates. {A}s such, they can be implemented by research and health centers having access to {C}ulturing, {DNA} isolation, and {PCR}.},
  keywords = {{C}ysteine protease {B} ; {L}eishmaniasis ; {T}rypanosomatidae ; {O}ligochromatography ; {D}ipstick ; {D}ifferential diagnosis},
  booktitle = {},
  journal = {{D}iagnostic {M}icrobiology and {I}nfectious {D}isease},
  volume = {63},
  numero = {2},
  pages = {173--181},
  ISSN = {0732-8893},
  year = {2009},
  DOI = {10.1016/j.diagmicrobio.2008.10.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010044248},
}